A tigress and its two cubs were found dead in Brahmapuri division of Maharashtra, in what is believed to be a case of poisoning.
The three bodies were recovered from near a drain in Metepur village under the Chimur forest range of Chandrapur district on Monday.
The tiger cubs are said to be around a year old.
The recovery of a partly eaten carcass of a spotted deer has raised suspicion that the Tigers were poisoned.
Chief Conservator of Forest S V Rama Rao said that an inquiry has been ordered to ascertain the cause of death.
Bramhapuri, which falls under Chandrapur, has around 40-60 tigers in addition to others who stray into the region from the Tadoba Andhari Tiger Reserve (TATR) roaming outside the protected area.
The region has a history of human-animal conflicts.
This year alone tigers have killed six people in Bramhapuri forest division. According to Brahmapuri Deputy Conservator of Forests, Kulraj Singh.
Brahmapuri division, which is spread over 986 sq km has over 610 villages, many of them close to the forest.
Tigers and leopards have regularly attacked cattle herds and at occasions humans who enter the forest in search of firewood.
Earlier this year, a tigress named E-1 and has been blamed for three human killings was captured and radio-collared to her movements can be monitored. The authorities have planned to release her at Melghat Tiger Reserve (MTR) in Amravati, despite earlier attempts to relocate tigers have repeatedly failed, often with the big cat dying an untimely death.